DNA SafeStain is a new and safe nucleic acid stain for the visualization of doublestranded DNA, single-stranded DNA, and RNA in agarose gels. The dyes are developed to replace toxic Ethidium Bromide (EtBr, a potent mutagen), commonly used in gel electrophoresis for visualization of nucleic acids in agarose gels. DNA SafeStain is noncarcinogenic by the Ames-test. The results are negative in both the mouse marrow chromophilous erythrocyte micronucleus and mouse spermary spermatocyte chromosomal aberration tests. DNA SafeStain emits green fluorescence when bound to DNA. It has two excitation wavelength peaks when bound to nucleic acid, at 290nm and 490nm. The emission wavelength for the DNA SafeStain is 530nm.